Protein preparation for NMR gives further details on how to optimise the buffer conditions for your sample. The acquisition of standard NMR spectra takes from a few minutes (1D spectra) to 3/4 days ...

Nuclear magnetic resonance (NMR) spectroscopy can be used to monitor the dynamic behavior of a protein at a multitude of specific sites. Moreover, protein movements on a broad range of timescales ...

While this is undoubtedly true of the many beautiful structures that have been produced both by X-ray crystallography and more recently by NMR spectroscopy ... the path for protein engineering ...