There is no noteworthy alternative fate for fatty acids in most bacteria, and all of the long-chain acyl-ACPs that are produced by the biosynthetic pathway are incorporated into the membrane.

First, PlsX catalyzes the synthesis of the intermediate fatty-acyl-phosphate (acyl-P) from acyl-ACP. Next, PlsY transfers the fatty acid from the activated acyl intermediate to the 1-position of G3P.