This configuration is ideal for in vivo imaging of whole specimens. The Ultima IV is equipped with a tunable two-photon excitation laser that allows dual color imaging of CFP/GFP/YFP together with ...

They placed a well-characterized substrate of PKA between blue and green mutants of GFP Phosphorylation of this ... reduced fluorescence energy transfer (FRET) between the two GFPs.

They found that although the imC cells did capture the secreted GFP resulting in a GFP gradient, the resulting gradient ...