PhyTips are sold with various affinity-based matrices, including protein A and G products for the purification of immunoglobulins, and products for the purification of His- and GST-tagged proteins.
Autoinduction in ZY and TYMV media, respectively, yielded about twice as much purified β-gal protein, between 1.6 and 1.7 times as much NusA, but 1.3 and 0.9 times as much GST as did IPTG ...
(D) GST and GST-AKR1A1s were purified and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. (E) AKR1A1 activity of the purified GST-AKR1A1s was determined. AKR1A1 produced by ...