At this point, RNA polymerase begins moving down the DNA template strand in the 3' to 5' direction ... which are slightly different than the deoxyribosemolecules found in DNA.
Discover methods for accurate DNA and RNA quantification and learn how to analyze purity and concentration to enhance ...
Denature the RNA by heating at 75 °C for 5 min, then rapidly ... 37 °C for 15 min, and then inactivate the terminal transferase by heating the reactions at 80 °C for 3 min.
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