Prior to the development of PCR in the 1980s, the primary method for producing many copies of a gene was a relatively time-consuming process known as DNA cloning. This technique involved insertion ...

PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of ...

PCR stands for polymerase chain reaction. It’s a multi-step process conducted in a machine that amplifies DNA through a series of temperature-controlled cycles. The entire process from start to ...

This technique allows scientists to see the results almost immediately while the process is still ongoing, whereas conventional RT–PCR only provides results at the end of the process. Real time RT–PCR ...

Polymerase chain reaction (PCR)-based rapid sterility testing offers a revolutionary alternative, delivering accurate contamination detection in hours while maintaining the sensitivity needed to ...

Overlay PCR reaction with 15 μl of mineral oil to ... rotate the to 96-well plate half way through the mixing process. Assess the quality of the emulsions at 400× magnification with an inverted ...

ABL1 digital PCR can reliably quantify stable deep molecular remission of chronic myeloid leukemia (CML), which will help to determine for which patients chronic drug treatment could potentially be ...

The addition of nivolumab to neoadjuvant chemotherapy significantly increased pathologic complete response among certain ...

Shesheer Kumar, co-founder of Huwel, explained how traditional RT-PCR testing involves the cumbersome DNA extraction step, where genetic material is isolated from a sample using a process called ...