Hybridize the DNA and RNA at the chosen temperature for 12–16 h. 13. Keeping the body of the tube submerged, open the lid of the hybridization tube. Rapidly add 300 μl of ice-cold nuclease S1 ...

The team observed a 4.4-fold increase in the editing efficiency of this modified nuclease, surpassing most of the commonly used RNA-guided endonucleases, including CjCas9. They called this variant ...

Wulf Blankenfeldt at HZI. "The nuclease Cas13b can recognize and cut RNA. It is currently used to silence genes, whether to study their function, clear viruses, or counteract genetic diseases ...

In one, we studied the structure and mechanism of activity of the catalytic RNA moiety of the RNA processing enzyme ribo-nuclease P (Rnase P). In the second, we explored naturally occurring ...

This powerful enzyme is nonspecific and highly active, capable of completely digesting RNA and DNA in various ... cell DNA was significantly reduced by nuclease treatment of the lentiviral ...

DNA can be duplicated or read to produce RNA, which is translated to generate protein. So ran the 'central dogma' of biology, devised by Francis Crick in 1958 to explain the directional flow of ...

We have solved the cryo-EM structure of AcrVIA1 bound to Cas13a crRNA, establishing that AcrVIA1 interacts with the guide-exposed face of Cas13a, thereby preventing access to the target RNA and the ...

To safeguard DNA and RNA from nonspecific cleavage by these molecular scissors, our cells support elegant mechanisms of regulation to ensure nucleases cut precisely. To uncover the mechanisms for ...

The firm will use the funds to develop its lead candidate in primary hyperoxaluria type 1 and other pipeline products in liver and CNS disorders.