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To obtain high densities of sgRNA that would give signals significantly ... The clusters are placed at least 5 Mbp away from each other (Figure 1A). In total, we selected 1 124 sgRNAs that are ...
designed and built to guide Cas9 to bind and cut desired intronic regions in the EML4 and ALK gene targets, trigger the paracentric genomic rearrangement event upon co-transfection (Figure 1).