Nov 10, 2022 · Reverse the cell flow path by swapping the inlet and outlet lines. If you suspect a clog or a partial obstruction of the cell, normally associated to an increase in pressure, follow …

It is very important to carefully select a proper flow cell for your HPLC system. You may need a high-pressure flow cell when several detectors – or a detector and a fraction collector – are …

HPLC flow cells are high-performance parts and key elements of the optical unit of a HPLC detector and part of the optofluidic flow path. Depending on the detector module and flow rate …

Oct 31, 2024 · If you want the best LC data, you need a properly working UV flow cell that is matched to the application. The primary goal is to choose the flow cell length that provides the …

Mar 16, 2011 · Modern UV/VIS detectors offer several different flow cell options. The option (s) you select can make a big difference in the level of signal sensitivity, sample dispersion and …

Nov 10, 2022 · Flush the system with HPLC-quality IPA with a flow rate of 1 mL/min for a minimum of 2 hours. Make sure that the pressure does not exceed 60 bars for UV, and 7 bars …

When leaving LC idle, pump a mobile phase with at least 5-10% of organic solvent. Observe the pressure limits of flow cells. Be careful when using detectors in series or fraction collectors.

Dec 20, 2021 · Start the flow at a very low rate, ensuring that the back pressure remains below the maximum rating (1000 psi for UV, 100 psi for RI) for the flow cell. Flush at a low flow for at …

Discover KNAUER’s flow cells for HPLC systems, designed for high-precision detection and ensuring reliable, reproducible results in your chromatography analysis.

Jan 16, 2023 · Overall, proper cleaning of the flow cell is critical to the success of your experiment. By following these guidelines and incorporating them into your routine …