A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by …

What is Myc-tag? Myc-tag is an epitope tag derived from the c-Myc protein. How does Myc-tag work? A Myc-tag can be used to detect expression of recombinant proteins. Therefore, the Myc-tag is genetically fused/cloned to a protein of interest (POI). After expression, the Myc-tagged protein can be captured and identified in crude biological samples.

The c-Myc (Myc) tag originated from c-myc, a proto-oncogene which encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis, and cellular transformation. The Myc tag is only 10 amino acids long (EQKLISEEDL).

May 11, 2023 · The Myc tag is a 10 amino acid peptide (EQKLISEEDL) derived from the protooncogene of the same name. The tag has a net negative charge with 4 negatively charged and 1 positively charged amino acids. Myc tags are frequently used for western blot, flow cytometry, and immunofluorescence .

Explore widely-used epitope tags, such as c-myc and HA, including recommended applications and what to watch out for.

The Myc tag is a small, immunoreactive peptide tag (11 amino acids) that is ideal for co-immunoprecipitation studies and Western blots. It binds to the cMyc Monoclonal Antibody and can be incorporated on the N-terminus, the C-terminus, or internally.

Jul 18, 2021 · The protein tag is a polypeptide or protein expressed as a fusion with the target protein using in vitro DNA recombinant technology, which facilitates the expression, detection, tracer and purification of the target protein.

2.1.3 c-Myc tag. The c-myc tag was created from the human c-myc gene product and is composed of 10 amino acids. This epitope tag is recognized by the anti-c-myc monoclonal antibody 9E10 [16]. The c-myc tag can be fused at the C- …

Apr 24, 2020 · The Myc and c-Myc tag are two terms for the same tag, which is derived from the c-Myc protein and has a peptide sequence of EQKLISEEDL. The tag is used to label target proteins to enable easier identification or purification.

ChromoTek Myc-Trap® is an effective optimized tool for immunoprecipitation (IP/Co-IP) and affinity purification of Myc-tagged proteins. Depending on whether you use a single Myc-tag, i.e. 1xMyc (EQKLISEEDL) or a double Myc-tag fused to the protein of interest, i.e. 2xMyc (EQKLISEEDL EQKLISEEDL) you can adjust your experiment.