Optical sectioning - Wikipedia
Optical sectioning is the process by which a suitably designed microscope can produce clear images of focal planes deep within a thick sample. This is used to reduce the need for thin sectioning using instruments such as the microtome.
Optical sectioning microscopy - Nature Methods
Nov 18, 2005 · Confocal scanning microscopy, a form of optical sectioning microscopy, has radically transformed optical imaging in biology. These devices provide a powerful means to eliminate from images...
ZEISS Microscopy Online Campus | Optical Sectioning Microscopy
To obtain crisp and sharp images, optical sections can be generated using either computational (deconvolution) or structured illumination techniques. This interactive tutorial explores the basic concept of optical sectioning using an animated cell model.
Optical sectioning microscopy with planar or structured …
Sep 29, 2011 · A key requirement for performing three-dimensional (3D) imaging using optical microscopes is that they be capable of optical sectioning by distinguishing in-focus signal from out-of-focus...
Optical sectioning microscopy - PubMed
We describe the core concepts of confocal microscopes and important variables that adversely affect confocal images. We also discuss data-processing methods for confocal microscopy and computational optical sectioning techniques that can perform optical …
Optical sectioning
Optical sectioning is the process by which a suitably designed microscope can produce clear images of focal planes deep within a thick sample. This is used to reduce the need for thin sectioning using instruments such as the microtome.
A phasor‐based approach to improve optical sectioning in any …
Jun 10, 2022 · We describe a method to boost the optical sectioning power of any confocal microscope. The method is based on the sequential acquisition of multiple confocal images acquired at different pinhole aperture sizes.
ZEISS Microscopy Online Campus | Optical Sectioning References
The ability to image thin sections without having to mechanically slice a thick specimen is afforded by optical sectioning microscopy. Sections are achieved by eliminating the excitation and detection of fluorescence that originates in regions removed from the focal plane.
Optical Sectioning Microscopy and Biological Imaging
In confocal microscopy, the crucial feature that enables optical sectioning is the use of a pinhole to reject emission light returned from areas outside the focal plane; in all forms of nonlinear microscopy, the optical sectioning is achieved by localized excitation.
Large-field optical sectioning structured illumination microscopy
In this study, we present a large-field optical-sectioning structured illumination microscopy (LF-OS-SIM) scheme, which features a large FOV and fast phase shifting. LF-OS-SIM utilizes a one-dimensional grating to generate stripe patterns and a spatial light modulator (SLM) to perform phase-shifting in the Fourier domain.
Computational optical sectioning with an incoherent multiscale …
Nov 4, 2021 · To address this problem, we propose an incoherent multiscale scattering model in a complete space for quantitative 3D reconstruction in complicated environments, which is called computational...
(PDF) Optical Sectioning Microscopy - ResearchGate
Jan 1, 2006 · We describe the core concepts of confocal microscopes and important variables that adversely affect confocal images. We also discuss data-processing methods for confocal microscopy and...
Nov 18, 2005 · Confocal scanning microscopy, a form of optical sectioning microscopy, has radically transformed optical imaging in biology. These devices provide a powerful means to eliminate from images the background caused by out-of-focus light and scatter.
Optical Sectioning-- Slices of Life | Science - AAAS
The development of the ability to collect a series of optical sections from multiply labeled living specimens by confocal microscopy, deconvolution, or multiple-photon imaging and to maintain registration between successive images has made multidimensional imaging a practical option.
Method of obtaining optical sectioning by using structured light …
We describe a simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object. Images taken at three spatial positions of the grid are processed in real time to produce optically sectioned images that are substantially similar to those obtained with confocal microscopes.
Optical Sectioning - (Modern Optics) - Fiveable
Optical sectioning is a technique used in advanced microscopy that allows for the visualization of thin slices or sections of a specimen, providing detailed images of structures at different depths.
Optical Sectioning - (College Physics I - Fiveable
Optical sectioning is a technique used in microscopy that allows for the visualization of thin, selective planes within a sample. It enables the imaging of specific layers or sections of a specimen, providing high-resolution, three-dimensional information about its internal structure.
High-definition imaging using line-illumination modulation
Mar 1, 2021 · Two-photon excitation microscopy (2PM) achieves better tissue penetration at an optical-sectioning ability close to that of confocal imaging by using near-infrared illumination, but...
Any way you slice it: a comparison of optical sectioning …
May 31, 2012 · Let’s look at three different kinds of confocal microscope, with a particular emphasis on how well they perform optical sectioning and the quality of the resulting images. The laser-scanning confocal is the “original” confocal, and can still be considered the gold standard, particularly for fixed samples.
Airborne Optical Sectioning - MDPI
Jul 4, 2018 · With airborne optical sectioning (AOS), we present a radically different approach that is based on an old idea: synthetic aperture imaging. Rather than measuring, computing, and rendering 3D point clouds or triangulated 3D meshes, …
Optical Sectioning - Java Tutorial | Olympus LS - Olympus …
This interactive Java tutorial explores optical sectioning using photomicrographs derived from specimens viewed under Hoffman modulation contrast illumination.
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