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  1. Nature
    https://www.nature.com › articles

    microRNAs in action: biogenesis, function and regulation

    • Ever since microRNAs (miRNAs) were first recognized as an extensive gene family >20 years ago, a broad community of researchers was drawn to investigate the universe of small regulatory RNA… See more

    Introduction

    It has been 30 years since curiosity-driven research into Caenorhabditis elegans development yielded the first insights into microRNAs (miRNAs)1,2, and their general regulatory … See more

    Nature
    High-throughput miRNA assays

    Until recently, direct experimental tests of miRNA biogenesis and function involved small to … See more

    Nature
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    Insights from RNase III cryo-EM structures

    The typically precise sequences of mature miRNAs that are hewn from long primary transcripts suggest that the stereotypical actions of Drosha and Dicer involve ruler-like measur… See more

    Nature
    Single-molecule imaging of miRNA factors

    Despite providing atomic-level information, cryo-EM and X-ray crystallography yield static snapshots that only indirectly inform molecular dynamics. A complementary approach i… See more

    Nature
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    Table of Contents
    Introduction
    High-throughput miRNA assays
    Insights from RNase III cryo-EM structures
    Single-molecule imaging of miRNA factors
     
  1. Studies of Pre Pri mRNA
    National Center for Biotechnology Information
    The Effects of Structure on pre-mRNA Processing and Stability
    Pre-mRNA molecules can form a variety of structures, and both secondary and tertiary structures have important effects on processing, function and stability of these molecules. The prediction of RNA secondary structure is a challenging problem and various algorithms that use minimum free energy, max...
    Author: Rachel Soeme…
    Published: 2017/08/08
    DOI: 10.1016/j.ymeth.2…
    PubMed
    Mechanisms and Regulation of Alternative Pre-mRNA Splicing
    Precursor messenger RNA (pre-mRNA) splicing is a critical step in the posttranscriptional regulation of gene expression, providing significant expansion of the functional proteome of eukaryotic organisms with limited gene numbers. Split eukaryotic genes contain intervening sequences or introns disru...
    Author: Yeon Lee
    Published: 2015
    Keywords: RNA struct…
    National Center for Biotechnology Information
    Pre-mRNA splicing: where and when in the nucleus
    Pre-mRNA splicing is essential for gene expression in mammalian cells in which most protein-coding genes are disrupted by intervening sequences (introns). The process to remove introns is efficient and precise, thus constituting the vast majority of constitutive splicing events in the cell. However,...
    Author: Joonhee Han, …
    Published: 2011/06
    DOI: 10.1016/j.tcb.201…
    National Center for Biotechnology Information
    Pre-mRNA modifications and their role in nuclear processing
    Non-coding RNAs, including ribosomal RNAs (rRNAs), transfer RNAs (tRNAs) and spliceosomal small RNAs (snRNAs), are extensively modified with more than 100 chemically distinct nucleotides that promote their activities in translation and splicing. The recent development of transcriptome-wide approache...
    Author: Nicole M. Marti…
    Published: 2018/09
    DOI: 10.1007/s40484-0…
    National Center for Biotechnology Information
    Pre-mRNA splicing-associated diseases and therapies
    Precursor mRNA (pre-mRNA) splicing is an essential step in human gene expression and is carried out by a large macromolecular machine called the spliceosome. Given the spliceosome's role in shaping the cellular transcriptome, it is not surprising that mutations in the splicing machinery can result i...
    Author: Sierra L. Love…
    Published: 2023
    Publication: RNA Biol…
  2. Nature
    https://www.nature.com › articles

    Pri-miRNA processing: structure is key | Nature …

    Jan 31, 2017 · In animal cells, pri-miRNAs are processed into pre-miRNAs by the Microprocessor complex in the nucleus, and are then transported into the …

    • Author: Louise Adams
    • Publish Year: 2017
  3. Nature
    https://www.nature.com › articles

    MicroRNA profiling: approaches and considerations

    Apr 18, 2012 · Biogenesis of mature miRNAs occurs through a multi-step process that begins with the cleavage of the primary miRNA (pri-miRNA) to yield an …

    • Author: Colin C. Pritchard, Heather H. Cheng, Heather H. Cheng, Muneesh Tewari
    • Publish Year: 2012
  4. Biology LibreTexts
    https://bio.libretexts.org › Bookshelves › …

    15.8: RNA Processing in Eukaryotes - mRNA Processing

    Oct 31, 2023 · Pre-mRNAs are first coated in RNA-stabilizing proteins; these protect the pre-mRNA from degradation while it is processed and exported out of the nucleus. The three most important steps of pre-mRNA processing are the …

  5. Biology LibreTexts
    https://bio.libretexts.org › Courses › Lum…

    12.5: pre-RNA and mRNA - Biology LibreTexts

    Feb 28, 2021 · Pre-mRNA splicing involves the precise removal of introns from the primary RNA transcript. The splicing process is catalyzed by protein complexes called spliceosomes that are composed of proteins and RNA …

  6. Images of Pre Pri mRNA

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  7. Science | AAAS
    https://www.science.org › doi

    Structural basis of pre-mRNA splicing | Science - AAAS

    Aug 20, 2015 · In eukaryotic cells, the coding exons in a freshly transcribed precursor messenger RNA (pre-mRNA) are interdispersed by noncoding introns that must be removed before protein translation.

  8. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov › pmc › articles

    The Effects of Structure on pre-mRNA Processing and Stability

  9. Wikipedia
    https://en.wikipedia.org › wiki › Primary…

    Primary transcript - Wikipedia

    The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor mRNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing. Pre …

  10. Springer
    https://link.springer.com › article

    Regulation of pri- MIRNA processing: mechanistic insights into the ...

  11. PubMed
    https://pubmed.ncbi.nlm.nih.gov

    Mechanisms and Regulation of Alternative Pre-mRNA Splicing