Jun 1, 2024 · TORCAR is a FRET-based mTORC1 sensor that reports mTORC1 activity upon GF stimulation by FRET signals at the single-cell level (Fig. S2J). However, neither insulin …

Plasmid pcDNA3-TORCAR from Dr. Jin Zhang's lab contains the insert TORCAR and is published in Cell Rep. 2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 10.1016/j.celrep.2015.02.031. …

Mar 17, 2015 · Here, we describe a genetically encoded mTORC1 activity reporter (TORCAR) that exhibits a change in FRET in response to phosphorylation by mTORC1. Co-imaging …

Nov 30, 2020 · Using a nuclear targeted Akt Substrate-based Tandem Occupancy Peptide Sponge (Akt-STOPS) that we developed for specific inhibition of Akt, a critical upstream …

To examine the spatial regulation of the activity of mTORC1 in live cells, we describe a protocol using a newly developed molecular tool, a genetically encoded fluorescence resonance …

Notes: Designed by Eric Greenwald and the Jin Zhang Lab at UCSD

Jun 16, 2022 · This observation was enabled by the development of a genetically encoded mTORC1 activity reporter (TORCAR). Briefly, the mTORC1 substrate 4EBP1 is sandwiched …

Sep 1, 2022 · In this study, we examined the expression of a 4E-BP1-based reporter (mTORC1 activity reporter; TORCAR) with various localization signal tags to clarify the relationship …

Mar 17, 2015 · Here, we describe a genetically encoded mTORC1 activity reporter (TORCAR) that exhibits a change in FRET in response to phosphorylation by mTORC1. Co-imaging …

Dec 7, 2016 · To examine the spatial regulation of the activity of mTORC1 in live cells, we describe a protocol using a newly developed molecular tool, a genetically encoded …